Additional Selective and Differential Cell Culture Media
NEOGEN Rose Bengal Chloramphenicol Agar, 500 g
Rose Bengal Chloramphenicol Agar is used for the selective isolation and enumeration of yeasts and molds from foods. Rose Bengal Chloramphenicol Agar is not intended for use in the diagnosis of disease or other conditions in humans. Rose Bengal Chloramphenicol Agar Base is a selective medium for the enumeration of fungi. In 1944, Smith and Dawson used Rose Bengal for the selective isolation of fungi from soil samples. This formula was prepared with a neutral pH, deviating from the usual acidified medium. Several investigations have found a neutral pH with the addition of a selective agent have been successful in supporting fungal growth and restricting bacterial growth. Rose Bengal Agar pre-supplemented with Chloramphenicol is a modification of the Rose Bengal Chlortetracycline Agar formula of Jarvis.For more information visit https://www.neogen.com/solutions/Microbiology/rose-bengal-chloramphenicol-agar
NEOGEN M-Green Yeast and Fungi Broth, 500 g
M-Green Yeast and Fungi Broth is used for the detection of fungi in beverages in a laboratory setting. M-Green Yeast and Fungi Broth is not intended for use in the diagnosis of disease or other conditions in humans.M-Green Yeast and Fungi Broth is a relatively more complex formula compared to other media used for isolation of fungi and yeast. This formulation is rich in nutrients which allows for excellent fungal growth. Bacterial growth is inhibited by an acid pH.Fungi have been found in potable water and on the inner surface of distribution system pipes. They can survive water treatment or they enter the system after treatment and remain viable.For more information visit https://www.neogen.com/solutions/Microbiology/m-green-yeast-and-fungi-broth
NEOGEN Rose Bengal Chloramphenicol Agar, 5 kg
Rose Bengal Chloramphenicol Agar is used for the selective isolation and enumeration of yeasts and molds from foods. Rose Bengal Chloramphenicol Agar is not intended for use in the diagnosis of disease or other conditions in humans. Rose Bengal Chloramphenicol Agar Base is a selective medium for the enumeration of fungi. In 1944, Smith and Dawson used Rose Bengal for the selective isolation of fungi from soil samples. This formula was prepared with a neutral pH, deviating from the usual acidified medium. Several investigations have found a neutral pH with the addition of a selective agent have been successful in supporting fungal growth and restricting bacterial growth. Rose Bengal Agar pre-supplemented with Chloramphenicol is a modification of the Rose Bengal Chlortetracycline Agar formula of Jarvis.For more information visit https://www.neogen.com/solutions/Microbiology/rose-bengal-chloramphenicol-agar
NEOGEN Dermatophyte Test Medium (DTM), 500 g
Dermatophyte Test Medium is used for the selective isolation of dermatophytic fungi and is not intended for use in the diagnosis of disease or other conditions in humans.In 1969, Taplin et al. developed this medium for the isolation and recognition of dermatophytic fungi, the causative agent of ringworm from hair, nails, and skin. Dermatophyte Test Medium is preferred for isolation and early recognition of Microsporum, Trichophyton, and Epidermophyton genera because of a distinct color change in the medium. Rapidly-growing species may produce a complete color change in the medium in 3 days. The slower-growing species will change the indicator in longer time periods. Other organisms may grow but can be recognized as non-dermatophytes by lack of a color change.For more information visit https://www.neogen.com/solutions/Microbiology/dermatophyte-test-medium-dtm
NEOGEN Pancreatic Digest of Gelatin, 500 g
Pancreatic Digest of Gelatin is an enzymatic digest of gelatin for use in preparing microbiological culture media in a laboratory setting. Pancreatic Digest of Gelatin is not intended for use in the diagnosis of disease or other conditions in humans.Pancreatic Digest of Gelatin provides a complex nitrogen source and is used as a media ingredient for fermentation studies. Pancreatic Digest of Gelatin alone is used to support the growth of non-fastidious microorganisms. Pancreatic Digest of Gelatin is deficient in carbohydrates and distinguished by low cystine and tryptophan content.For more information visit https://www.neogen.com/solutions/Microbiology/pancreatic-digest-gelatin
NEOGEN Pancreatic Digest of Gelatin, 5 kg
Pancreatic Digest of Gelatin is an enzymatic digest of gelatin for use in preparing microbiological culture media in a laboratory setting. Pancreatic Digest of Gelatin is not intended for use in the diagnosis of disease or other conditions in humans.Pancreatic Digest of Gelatin provides a complex nitrogen source and is used as a media ingredient for fermentation studies. Pancreatic Digest of Gelatin alone is used to support the growth of non-fastidious microorganisms. Pancreatic Digest of Gelatin is deficient in carbohydrates and distinguished by low cystine and tryptophan content.For more information visit https://www.neogen.com/solutions/Microbiology/pancreatic-digest-gelatin
NEOGEN Lysine Iron Agar, 5 kg
Lysine Iron Agar is used for the differentiation of microorganisms on the basis of lysine decarboxylase and hydrogen sulfide production. Lysine Iron Agar is not intended for use in the diagnosis of disease or other conditions in humans. Lysine Iron Agar is prepared according to the formulation of Edwards and Fife, who developed the medium to detect Salmonella arizonae. S. arizonae ferments lactose rapidly, and the authors found expected H2S production on Triple Sugar Iron Agar was suppressed. Detection of S. arizonae is important because it has been implicated in food borne infections. By eliminating lactose and incorporating lysine, Edwards and Fife devised a medium differentiating enteric bacilli based on their ability to decarboxylate or deaminate lysine and produce abundant hydrogen sulfide. This medium is recommended for detecting rapid lactose-fermenting S. arizonae. For more information visit https://www.neogen.com/solutions/Microbiology/lysine-iron-agar
NEOGEN LB Agar, Miller, 10 kg
LB Agar (Miller), a nutritionally rich medium designed for growth of pure cultures of recombinant strains, is based on the formulation described by Miller. E. coli is grown to late log phase in LB Medium. Some plasmid vectors replicate to a high copy number and do not require selective amplification. Some vectors do not replicate so freely and need to be selectively amplified. Chloramphenicol may be added to inhibit host synthesis and prevent replication of the bacterial chromosome.LB Agar, Miller contains 10 g/L of sodium chloride, different from the levels in Lennox formulations. This allows the researcher to select the optimal salt concentration for a specific strain. The medium may be aseptically supplemented with glucose or antibiotics.For more information visit https://www.neogen.com/solutions/Microbiology/lb-agar-miller
NEOGEN Tetrathionate (TT) Broth Base, Hajna, 500 g
Tetrathionate (TT) Broth Base, Hajna is used as a selective enrichment for the cultivation of Salmonella species (except S. typhi) from samples in a laboratory setting. Tetrathionate (TT) Broth Base, Hajna is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base, Hajna, is recommended in the Compendium of Methods for the Microbiological Examination of Foods and the FDA/BAM for the Environmental Sampling and Detection of Salmonella Enteritidis (SE) in Poultry Houses and whole eggs.. Salmonella organisms may be present but injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. Numerous reported cases of Salmonella enteritidis (SE) infections are associated with contaminated shell eggs.For more information visit https://www.neogen.com/solutions/Microbiology/tetrathionate-tt-broth-base-hajna
NEOGEN Letheen Broth Base without Tween, 5 kg
Letheen Broth Base (no tween) is used with Tween 80 (Polysorbate 80) for the testing of samples containing quaternary ammonium compounds for antimicrobial activity in a laboratory setting. Letheen Broth Base (no tween) is not intended for use in the diagnosis of disease or other conditions in humans.In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds while testing germicidal activity. Letheen Agar is a modification of TGE Agar with the addition of Lecithin and Polysorbate 80.Letheen Broth Base was developed as a subculture medium for the neutralization of quaternary ammonium compounds in disinfectant testing.For more information visit https://www.neogen.com/solutions/Microbiology/letheen-broth-base-without-tween
NEOGEN Tryptose Phosphate Broth, 10 kg
Tryptose Phosphate Broth is used for the cultivation of a wide variety of fastidious microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans. This is a versatile, nutritionally rich buffered glucose broth. The medium is a general-purpose broth that has been used as a blood culture medium and with the addition of sodium azide 0.025% as a selective medium for streptococci in dairy products.For more information visit https://www.neogen.com/solutions/Microbiology/tryptose-phosphate-broth
NEOGEN Glucose OF Medium, 500 g
Glucose OF Medium is used for the confirmation of Enterobacteriaceae in foods according to ISO 21528-1&2:2017, and is not intended for use in the diagnosis of disease or other conditions in humans.Enzymatic digest of casein provides nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dipotassium phosphate acts as a buffer system. Glucose is the fermentable carbohydrate providing carbon and energy. Bromothymol blue is the pH indicator.Colonies of presumptive Enterobacteriaceae should be confirmed by means of tests for the fermentation of glucose and the presence of oxidase. If the colonies are oxidase-negative and glucose-positive, the sample shall be regarded as being positive for Enterobacteriaceae.For more information visit https://www.neogen.com/solutions/Microbiology/glucose-of-medium
NEOGEN Glucose OF Medium, 5 kg
Glucose OF Medium is used for the confirmation of Enterobacteriaceae in foods according to ISO 21528-1&2:2017, and is not intended for use in the diagnosis of disease or other conditions in humans.Enzymatic digest of casein provides nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dipotassium phosphate acts as a buffer system. Glucose is the fermentable carbohydrate providing carbon and energy. Bromothymol blue is the pH indicator.Colonies of presumptive Enterobacteriaceae should be confirmed by means of tests for the fermentation of glucose and the presence of oxidase. If the colonies are oxidase-negative and glucose-positive, the sample shall be regarded as being positive for Enterobacteriaceae.For more information visit https://www.neogen.com/solutions/Microbiology/glucose-of-medium
NEOGEN Phenylethanol Agar, 500 g
Phenylethanol Agar is used with blood for the selective isolation of Gram-positive cocci in a laboratory setting. Phenylethanol Agar is not intended for use in the diagnosis of disease or other conditions in humans. Brewer and Lilley reported the addition of phenylethanol to a nutritive medium permitted growth of Gram-positive organisms, but markedly to completely inhibited growth of Gram-negative organisms. Phenylethanol Agar inhibits swarming of Proteus spp., and can be used to selectively isolate anaerobic bacteria from specimens with mixed flora. Phenylethanol Agar is specified for use in several reference methods.For more information visit https://www.neogen.com/solutions/Microbiology/phenylethanol-agar
NEOGEN Tryptone Glucose Extract (TGE) Agar, 5 kg
Tryptone Glucose Extract (TGE) Agar is used for the cultivation and enumeration of microorganisms in dairy and bottled water products. Tryptone Glucose Extract (TGE) Agar is not intended for use in the diagnosis of disease or other conditions in humans. In the 1930’s, Bower and Hucker developed a medium for detecting bacteria in milk and other dairy products. Prickett used a glucose agar containing tryptone to study thermophilic bacteria in milk. This medium is known as Yeast Dextrose Agar. In 1948, the American Public Health Association (APHA) adopted Tryptone Glucose Extract Agar for use in testing milk and dairy products. Currently, APHA specifies Tryptone Glucose Extract Agar for the heterotrophic plate count procedure in testing bottled water. Tryptone Glucose Extract Agar is also known as Yeast Dextrose Agar.For more information visit https://www.neogen.com/solutions/Microbiology/tryptone-glucose-extract-tge-agar